The Cybertongue® Company

Testing Services

PPB Technology currently offers testing services for the following proteases and protease precursors (zymogens):

AprX

AprX is a protease produced by psychrotropic bacteria, such as Pseudomonas fluorescens. Ultra heat treatment (UHT) processes kill the bacteria but some AprX protease remains. The residual AprX can digest milk proteins and cause UHT milk to spoil before its use-by-date. PPB Technology can measure AprX activity at levels of concern in raw and processed milk.

Plasmin

Plasmin is a protease endogenous to cow milk. The health and age of cows affects the amount of plasmin in milk. Not all plasmin is removed during UHT processing and, like AprX, the remaining plasmin may spoil UHT products before their use-by-dates. PPB Technology has an assay to measure plasmin activity in raw and processed milk.

Plasminogen

Plasminogen is the (zymogen) precursor to plasmin. Plasminogen is produced by the cow and can be converted into plasmin, which damages milk proteins as described above. The plasmin test measures the current level of active plasmin in milk, but not the potential for plasminogen to convert to plasmin over time. PPB Technology can measure the total plasminogen in milk as well as the active plasmin. This may help predict future shelf-life problems.

Bacillus Proteases

In Development

Thermoduric bacteria form spores under the heating and drying conditions used to make powdered milk. When powdered milk is reconstituted, the spores may germinate and secrete aggressive proteases that degrade milk quality. PPB Technology is developing a test for proteases produced by thermoduric Bacillus spp. If you would like to be involved in pre-commercial trials of a Bacillus protease assay, please contact PPB Technology in confidence.

Using PPB Technology’s Testing Services

  • Contact us to discuss the scope of the testing and for a quote.
  • PPB Technology will send you a sampling kit including approved sample tubes.
  • You collect and freeze samples and ship them to us frozen, by courier. (For overseas customers, we will provide you with an import permit to accompany the shipment).
  • We will provide you with test results and interpret them for you based on our proprietary milk spoilage models.
PPB Technology currently accepts samples of bovine milk from Australia and a number of other countries.
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australia

austria

belgium

canada

chile

czech republic

denmark

estonia

finland

france

germany

hungary

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indonesia

ireland

italy

japan

latvia

lithuania

luxembourg

malta

mexico

netherlands

new caledonia

new zealand

norway

poland

portugal

romania

singapore

slovakia

slovenia

spain

sweden

switzerland

united kingdom

united states of america

vanuatu