The Cybertongue® Company

PPB Technology’s Peer-Reviewed Paper on the Cybertongue Biosensor for AprX Protease of Pseudomonas fluorescens Has Been Published

Our peer-reviewed paper on the #CYBERTONGUE biosensor for AprX protease of Pseudomonas fluorescens has just been published.

Here are the highlights:

  • A BRET-based biosensor was designed to measure the activity of the AprX metalloprotease.
  • The biosensor incorporates a synthetic protease recognition site.
  • This biosensor is approximately 800x more sensitive than the established method and highly selective for AprX over other proteases found in milk.
  • The biosensor was calibrated to measure AprX activity in enzyme units.
  • The biosensor enables dairy processors to mitigate heat-stable proteases in milk. 

The research described in the paper started within the CSIRO and continued in a collaboration between the CSIRO and PPB Technology Pty Ltd. It is published in Analytica Chimica Acta and is open source for all to read.

Thank you to all the co-authors, including Helen DacresFelix Weihs, Jian Wang and Alisha Anderson

Read it here: