Our peer-reviewed paper on the #CYBERTONGUE biosensor for AprX protease of Pseudomonas fluorescens has just been published.
Here are the highlights:
- A BRET-based biosensor was designed to measure the activity of the AprX metalloprotease.
- The biosensor incorporates a synthetic protease recognition site.
- This biosensor is approximately 800x more sensitive than the established method and highly selective for AprX over other proteases found in milk.
- The biosensor was calibrated to measure AprX activity in enzyme units.
- The biosensor enables dairy processors to mitigate heat-stable proteases in milk.
The research described in the paper started within the CSIRO and continued in a collaboration between the CSIRO and PPB Technology Pty Ltd. It is published in Analytica Chimica Acta and is open source for all to read.
Read it here: https://lnkd.in/g5fAd9zk